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We offer four basic Starter/Developer Kits for rapid ELISA development (with universal SA-PolyHRP product line) and five different Blotting Kits. ♦ SA-PolyHRP Starter Kit #SP-START01 (12* × 96) - €191,60 #SP-START01 is a minimal version of trial/developer packthat offers enough SA-PolyHRP conjugates for running at least 12* complete 8 × 12 ELISA plates * given number of plates suggests using Streptavidin-PolyHRP conjugates in working dilution 1/5.000 (from 1 mg/ml stock, corresponds to effective conjugate concentration 200 ng/ml). Said dilution is recommended as starting dilution in development of HS ELISA test systems that will be fairly close to a maximum final working strength dilution in many HS ELISAs. Higher dilutions up to 1/10.000 - 1/20.000 are frequently been applied in many ultrasensitive ELISA systems with strong matched antibody pairs. #SP-START01 includes three basic SA-PolyHRP items in pre-diluted stabilized 1/500 format (2 µg/ml): 1) 25 µg/12,5ml SA-PolyHRP20 #SP20D2 (€93,10) 2) 25 µg/12,5ml SA-PolyHRP40 #SP40D2 (€93,10) 3) 25 µg/12,5ml SA-PolyHRP80 #SP80D2 (€93,10) (∑ = €279,30) You save €87,70 and get a possibility of trying all three SA-PolyHRPs for only €191,60 provided you will be using your own ancillary diluents/blockers that are well compatible with SA-PolyHRP. Since the latter is rather uncertain assumption we strongly recommend to begin with ♦♦ SA-PolyHRP Starter Kit #SP-START1 (12 × 96) - €346,00 This kit additionally contains certificated diluents/blockers that have been developed especially for using with SA-PolyHRP and therefore are ideally compatible with SA- PolyHRP conjugates. #SP-START1 contains the following universal detecting and auxiliary reagents: 1) 25 µg/12,5ml SA-PolyHRP20 #SP20D2 (€93,10) 2) 25 µg/12,5ml SA-PolyHRP40 #SP40D2 (€93,10) 3) 25 µg/12,5ml SA-PolyHRP80 #SP80D2 (€93,10) 4) 250 ml Universal (SA-PolyHRP) Casein Diluent/Blocker, #UCDB (€75,40) as SA-PolyHRP Diluent and for Blocking plates after coating with capture antibody 5) 250 ml Antibody/Antigen/(PolyHRP)Conjugate Diluent/Blocker #AADB (€52,90) as Sample/Calibrator Diluent and biotinylated anti-Analyte (Detector Antibody or Antigen) Diluent 6) 125 ml s(HS)TMB #sTMB (€51,80) 7) 65 ml Stop Solution #SER (€12,40) (∑ = €471,80) You save €125,80 while this kit will guarantee the best results you can obtain with PolyHRP in ELISA provided your specific reagents are good enough (refer to our PolyHRP Users Guide to check for applicable quality of your given specific reagents). ♦♦♦ SA-PolyHRP Starter Kit #SP-START2 (12 × 96) - €447,40 This kit has the same basic composition as #SP-START1 that is extra added with Coating and Wash Buffer Concentrates. The only materials you need to begin working with this "lazy student" version are just your specific antigen/antibody reagents, ELISA plates and accessories and water. 1) 25 µg/12,5ml SA-PolyHRP20 #SP20D2 (€93,10) 2) 25 µg/12,5ml SA-PolyHRP40 #SP40D2 (€93,10) 3) 25 µg/12,5ml SA-PolyHRP80 #SP80D2 (€93,10) 4) 250 ml Universal (SA-PolyHRP) Casein Diluent/Blocker, #UCDB (€75,40) as SA-PolyHRP Diluent and for Blocking plates after coating with capture antibody 5) 250 ml Antibody/Antigen/(PolyHRP)Conjugate Diluent/Blocker #AADB (€52,90) as Specimen/Calibrator Diluent and biotinylated anti-Analyte (Detector Antibody or Antigen) Diluent 6) 125 ml s(HS)TMB #sTMB (€51,80) 7) 65 ml Stop Solution #SER (€12,40) 8) 100 ml Binding/Coating Buffer 10X Concentrate #BBC (€37,20) 9) 500 ml Wash Buffer 10X Concentrate #WBC (€99,20) (∑ = €608,20) Next version is the larger ♦♦♦♦ SA-PolyHRP Developer Kit #SP-START3 (48 × 96) - €869,10 #SP-START3 contains enough reagents for running at least 48 complete 8 × 12 ELISA plates: 1) 100 µg/50ml SA-PolyHRP20 #SP20D2 (€273,00) or 100 µg/10 ml SA-PolyHRP20 #SP20D10 (€248,10) or 100 µg/2 ml SA-PolyHRP20 #SP20D50 (€148,90) 2) 100 µg/50ml SA-PolyHRP40 #SP40D2 (€273,00) or 100 µg/10 ml SA-PolyHRP40 #SP40D10 (€248,10) or 100 µg/2 ml SA-PolyHRP40 #SP40D50 (€148,90) 3) 100 µg/50ml SA-PolyHRP80 #SP80D2 (€273,00) or 100 µµg/10 ml SA-PolyHRP80 #SP80D10 (€248,10) or 100 µg/2 ml SA-PolyHRP80 #SP80D50 (∑148,90) 4) 1000 ml Universal (SA-PolyHRP) Casein Diluent/Blocker, #UCDB (€250,60) - as SA- PolyHRP Diluent and for Blocking plates after coating with capture antibody 5) 1000 ml Antibody/Antigen/(PolyHRP)Conjugate Diluent/Blocker #AADB (€175,40) - as Sample/Calibrator Diluent and biotinylated anti-Analyte (Detector Antibody or Antigen) Diluent 6) 500 ml s(HS)TMB #sTMB (€147,00) 7) 250 ml Stop Solution #SER (€44,60) (∑ = €1.402,60 - €1.041,30) Specific concentration/volume (pre-dilution) format of stabilized SA-PolyHRPs enclosed with the kit is the parameter of free developers' choice that has no effect on the kit price. This results in three extensions of the kit given in three different specific order codes which are as follows: #SP-START3-D2 contains all conjugates in 100µg/50ml (1/500, 2 µg/ml) format #SP-START3-D10 contains all conjugates in 100µg/10ml (1/100, 10 µg/ml) format #SP-START3-D50 contains all conjugates in 100µg/2ml (1/20, 50 µg/ml) format. We offer four different Blotting kits. ♦ SA-PolyHRP Starter Kit #SP-BLOT ~50* - €786,00 #SP-BLOT is designed for using with biotinylated primary antibodies from different species (mouse monoclonal antibodies, polyclonal antibodies from rabbit/ship/goat, etc., biotinylated) and contains all three Streptavidin-PolyHRP conjugates in pre-diluted stabilized 1/20 format (at 50 µg/ml), auxiliary Diluents/Blockers and ep(HS)TMB-mA in quantities enough for running at least (≥)50* blotting assays with larger (approx. 110-120 × 4-5 mm) membrane strips, all ~50 with either SA-PolyHRP20, SA- PolyHRP40 or SA-PolyHRP80, whatever the conjugate of your choice (after initial experimental evaluation in first starter runs) would finally be. * based on the assumption of using 2,5 ml volume of working reagent per strip/tray and maximum recommended working strength dilution of SA-PolyHRPs for blotting which is 1/500 (from 1 mg/ml stock); in many systems working dilution of conjugate will be essentially higher than 1/500 This kit contains certificated diluents/blockers that have been developed especially for using with SA-PolyHRP and therefore are ideally compatible with SA-PolyHRP detection. #SP-BLOT contains the following universal detecting and auxiliary reagents: 1) 250 µg/5ml SA-PolyHRP20 #SP20D50 (€310,20) 2) 250 µg/5ml SA-PolyHRP40 #SP40D50 (€310,20) 3) 250 µg/5ml SA-PolyHRP80 #SP80D50 (€310,20) 4) 250 ml Universal (SA-PolyHRP) Casein Diluent/Blocker, #UCDB (€75,40) as SA-PolyHRP Diluent and for Blocking membranes after antigen transfer 5) 140 ml Antibody/Antigen/(PolyHRP)Conjugate Diluent/Blocker #AADB (€23,10 × 1,4 = €32,30) - as Diluent for biotinylated anti-Analyte (primary Detector Antibody-biotin Conjugate) 6) 125 ml ep(HS)TMB-mA #epTMB-mA (€127,70) (∑ = €1.166,00) #SP-BLOT kit can also be used in ELISPOT and microchip/microarray applications including assays on non-membrane and non-porous carrier matrices. ♦♦ GAM-PolyHRP Developer kit, #GAMP-BLOT ~40* - €536,90 #GAMP-BLOT is designed for using with intact mouse (monoclonal) primary antibodies, also with non-purified mouse acsites fluid and cell culture/factory supernatants, and includes Goat anti-Mouse IgG (H+L)-PolyHRP40 in pre-diluted stabilized 1/20 format (50 µg/ml) along with Antibody/Antigen/(PolyHRP)Conjugate Diluent/Blocker and p(HS) TMB in quantities enough for running at least (≥)40* blotting assays with larger (approx. 110-120 × 4-5 mm) membrane strips. * based on the assumption of using 2,5 ml working reagent volume per strip/tray and near to recommended maximum (for blotting) working strength dilution of GAM-PolyHRP 1/200 (from 1 mg/ml stock); in many systems working dilution of conjugate will be essentially higher than 1/200, we have available e.g. customers' results with conjugate working well in dilution 1/5.000 #GAMP-BLOT contains the following reagents: 1) 500 µg/10ml GAM-PolyHRP40 #GAMP40D50 (€471,50) 2) 3 × 100 ml Antibody/Antigen/(PolyHRP)Conjugate Diluent/Blocker #AADB (€23,10 × 3 = €69,30) - as membrane Blocker and Diluent for both primary antibody and secondary GAM-PolyHRP Conjugate 3) 125 ml ep(HS)TMB-mA #epTMB-mA (€127,70) (∑ = €668,50) ♦♦♦ GAR-PolyHRP Developer kit, #GARP-BLOT ~40* - €536,90 #GARP-BLOT is designed for using with intact rabbit primary antibodies, either affinity purified, DEAE-purified IgG, partially purified (NH4/K-sulfate precipitated) γ-globulin fraction or non-purified rabbit antiserum, and includes Goat anti-Rabbit IgG (H+L)-PolyHRP40 in pre-diluted stabilized 1/20 format (50 µg/ml) along with Antibody/Antigen/(PolyHRP)Conjugate Diluent/Blocker and p(HS) TMB in quantities enough for running at least (≥)40* blotting assays with longer (approx. 110-120 × 4-5 mm) membrane strips. * based on the assumption of using 2,5 ml working reagent volume per strip/tray and near to maximum (blotting) working strength dilution of GAR-PolyHRP 1/200 (from 1 mg/ml stock); in many systems working dilution of conjugate will be essentially higher than 1/200 #GARP-BLOT contains the following reagents: 1) 500 µg/10ml GAR-PolyHRP40 #GARP40D50 (€471,50) 2) 3 × 100 ml Antibody/Antigen/(PolyHRP)Conjugate Diluent/Blocker #AADB (€23,10 × 3 = €69,30) - as membrane Blocker and Diluent for both primary antibody and secondary GAR-PolyHRP Conjugate 3) 125 ml ep(HS)TMB-mA #epTMB-mA (€127,70) (∑ = €668,50) ♦♦♦♦ PrA-PolyHRP Developer kit, #AP-BLOT ~40* - €536,90 #AP-BLOT is designed for using with intact primary antibodies from different mammalian species (Rabbit, Cow, Guinea pig, Pig, Dog, Mouse IgG1, IgG2a, IgG2b), either affinity purified, DEAE-purified IgG, partially purified (NH4/K-sulfate precipitated) γ-globulin fraction or non-purified antiserum, and includes recombinant Protein A - PolyHRP40 in pre-diluted stabilized 1/20 format (50 µg/ml) along with Antibody/Antigen/(PolyHRP) Conjugate Diluent/Blocker and ep(HS)TMB-mA in quantities enough for running at least (≥)40* blotting assays with longer (approx. 110-120 × 4-5 mm) membrane strips. * based on the assumption of using 2,5 ml working reagent volume per strip/tray and near to maximum (blotting) working strength dilution of PrA-PolyHRP 1/200 (from 1 mg/ml stock); in many systems working dilution of conjugate will be essentially higher than 1/200 #AP-BLOT contains the following reagents: 1) 500 µg/10ml PrA-PolyHRP40 #AP40D50 (€471,50) 2) 3 × 100 ml Antibody/Antigen/(PolyHRP)Conjugate Diluent/Blocker #AADB (€23,10 × 3 = €69,30) - as membrane Blocker and Diluent for both primary antibody and secondary PrA-PolyHRP Conjugate 3) 125 ml ep(HS)TMB-mA #epTMB-mA (€127,70) (∑ = €668,50) ♦♦♦♦♦ MMAH-PolyHRP Developer kit, #MAHGP-BLOT ~40* - €345,10 #MAHGP-BLOT is a different type of product. While an intended use of the first three blotting kits is primarily with research applications where target analyte is an antigen, non- specifically (via essentially "physical adsorption") immobilized onto membrane (usually after PAGE separation) from complex proteinacous mixtures and then detected through interaction with primary and further enzyme-labeled secondary (anti-species) antibody, #MMAHP-BLOT is designed for diagnostic serological Western-blot applications, where known natural (after PAGE) or recombinant/synthetic antigens are localized in predetermined band/spot positions onto reproducibly fabricated membrane. Said membrane with an array of antigens usually representative of specific pathogen (in infectious serological diagnostics) is brought into reaction with patient's serum. Binding of specific antibodies, if present, in characteristic membrane zones, visualized via anti-species (IgG) enzymatic conjugate and precipitating substrate, is indicative of infection or moreover of its specific development/progression stage. #MMAHP-BLOT includes monoclonal Mouse anti-human IgG (H, γ-chain specific) - PolyHRP40 in pre-diluted stabilized 1/20 format (50 µg/ml) along with Antibody/Antigen/(PolyHRP)Conjugate Diluent/Blocker and p(HS) TMB in quantities enough for running at least (≥)40* blotting assays with longer (approx. 110-120 × 4-5 mm) membrane strips. * based on the assumption of using 2,5 ml working reagent volume per strip/tray and near to maximum (blotting) working strength dilution of MAHG-PolyHRP 1/200 (from 1 mg/ml stock); in many systems working dilution of conjugate will be essentially higher than 1/200, one customer reported very good performance when utilizing conjugate diluted down to 1/2.500 #MMAHP-BLOT contains the following reagents: 1) 500 µg/10ml MAHG-PolyHRP40 #MAHGP40D50 (€259,40) 2) 3 × 100 ml Antibody/Antigen/(PolyHRP)Conjugate Diluent/Blocker #AADB (€23,10 × 3 = €69,30) - as membrane Blocker and Diluent for both Specimen (Serum/Plasma, others) and detecting MMAH-PolyHRP40 Conjugate 3) 125 ml ep(HS)TMB-mA #epTMB-mA (€127,70) (∑ = €456,40) #MMAHP-BLOT kit can also be used in IHC applications (autoimmune disorders/syndromes). |