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We currently offer two groups of products 1. SA-PolyHRP Diluents/Blockers and Stabilizers and 2. Antibody/Antigen and General Conjugate Diluents/Blockers and Stabilizers Click here for a structural scheme giving general product view. Both groups include ready-to-use products (balanced Diluents/Blockers and Stabilizers) and Casein Buffer Concentrates (CBC), intermediate products, that can be further processed into customer-tailored reagent having desired functionality. further processed into customer-tailored reagent having desired functionalitys. that had been developed for using in the capacity of auxiliary liquid reagents with SA- PolyHRP conjugates. Casein Buffer 20X-4X Concentrate 1 #CBC1 is a concentrated stable liquid filterable formulation of universal Casein Colloid Buffer, true biotin free, made in proprietary manufacturing process. Recommended use includes (I) Blocking of the solid phase (immunoplates, blotting membranes, other porous and non-porous materials) after coating with capture reagent (e.g. binding antibody or antigen) made with the sense of occupying free vacant sites onto immunosorbent surface. As a rule adsorbed inert blocker also supports immobilized specific capture reagent improving that way long-term stability of prepared immunosorbent, especially after drying. (II) Application of CBC as constituent part of diverse Diluent formulations with both specimen/calibrator and detector reagents/conjugates. Casein will eliminate adverse matrix effects with different complex specimens and block non specific binding (NSB) of detecting conjugates to immunosorbent in absence of analyte. 1 volume of CBC mixed with 3-to-19 volumes of your own diluent or simple PBS will yield 4-to-20 volumes of Casein Diluent/Blocker depending on desired working (NSB- eliminating) strength. CBC will effectively eliminate NSB/backgrounds in ELISA, blotting, ICT and IHC applications. Notice, that casein will block protein-to-protein binding of truly non- specific origin. CBC will not prevent specific interactions associated with e.g. heterophilic antibodies, HAMA, rheumatoid factors and other essentially specific interfering substances that may also cause false positive reactions in certain assay systems. (In order to eliminate above adverse, primarily human blood serum/plasma matrix effects add your Specimen/Sample diluent with Universal Blocker of heterophilic antibodies, rheumatoid factors, HAMA and complement #UB or HAMA Blocker #HAMAB). CBC1 is absolutely biotin-free and therefore is ideally compatible with enhanced biotin-streptavidin detection systems showing no drop in detection activity with streptavidin conjugates. Moreover, diluents made of CBC boost positive signals in systems with Streptavidin-PolyHRP conjugates which, along with lowering backgrounds, significantly improves signal-to-noise ratios. Each batch of produced CBC1 passes rigorous control providing evidence of complete endogenous biotin removal. SA-PolyHRP80 conjugate diluted 1/20.000 (50 ng/ml total conjugate, approx. 10 ng/ml conjugated Streptavidin) in 1/2 CBC1, after longer overnight exposition in diluted state, is applied in our standard ELISA QC release use test. Batch is released for sale if it does not show in above functional test quantitatively trustworthy inhibition effect in direct comparison with internal gold standard CBC1 reference (exhaustively biotin-depleted) material. Notice, that equimolar (free, 244.3 Da) biotin concentration that theoretically shall cause 100% inhibition in described situation (assuming streptavidin size 60 kDa and 4 active biotin-binding sites) is 162,86 pg/ml. This almost exactly corresponds to standard certification of vitamin-free caseins given typically 0,05 µg D-biotin per 1 g dry material. CBC used as a component of liquid ready-to-use formulations features stabilizing effect with detecting streptavidin & antibody conjugates and controls. CBC is especially useful in stabilizing larger PolyHRP conjugates. CBC is stable at +4°C at least 2 years from the date of manufacture. CBC1 is essentially 5.5% (w/v) 0.45µm/0.2µm-filterable colloidal solution of true biotin- free casein in 0,15M salt buffer base pH 7,3. It contains non-mercury safe wide- spectrum anti-microbial preservative/biocide 5-Bromo-5-Nitro-1,3-dioxane at 0,1%. CBC is filtered through 0.45µm at the end of manufacture process. Casein
Buffer 20X-4X Concentrate 1 Universal Casein Diluent/Blocker #UCDB is ready-to-use reagent applicable for blocking ELISA plates and blotting membranes, diluting complex specimens and diverse detection reagents/conjugates including high performance and ultrasensitive biotin-streptavidin systems. Effectively eliminates NSB in ELISA, blotting, ICT and IHC applications. UCDB is essentially a liquid formulation of 1,375 (w/v) biotin-free casein in neutral NaCl/Phosphate Buffer preserved with 5-Bromo-5-Nitro-1,3-Dioxane at 625 ppm, 0.45µm-filtered. UCDB is stable at +4°C over 2 years from the date of release. Universal (SA-PolyHRP) Casein Diluent/Blocker biotin-free, ready-to-use, #UCDB pricing 2. A second group, General Antibody/AntigenConjugate Diluents/Blockers and Stabilizers, consists of similarly designed casein and casein-based products with matching to first group functional background-eliminating and stabilizing activities. The only essential difference is presence of biotin which makes these products incompatible with SA-PolyHRP conjugates. Better to say not directly compatible" since non biotin free blocking and stabilizing buffers can still be unrestrictedly used with other components of diverse assay systems utilizing SA-PolyHRP where their functional performance will basically be well compared to performance of respective products of the first, biotin-free group. In sense of corresponding performance, by ruling out immediate use with SA-PolyHRP (as e.g. diluent or stabilizer), Casein Buffer 20X-4X Concentrate 2 #CBC2 is essentially equal to Casein Buffer 20X-4X Concentrate 1 #CBC1; Antibody/Antigen/(PolyHRP) ConjugateDiluent/Blocker #AADB is essentially equal to Universal (SA-PolyHRP) Casein Diluent/Blocker #UCDB; Antibody/Antigen (PolyHRP) Conjugate Stabilizer #AA1 is essentially equal to Streptavidin-PolyHRP Conjugate Stabilizer #SA1 and High Temperature Antibody/Antigen (PolyHRP) Conjugate Stabilizer HT #AA3-RHT is essentially equal to High Temperature Streptavidin-PolyHRP Conjugate Stabilizer HT #SA3-RHT. Therefore actually all said above about specialty biotin-free items equally applies to second group products, at least in what concerns basic composition and functional performance characteristics (with natural reservation concerning incompatibility with SA-PolyHRP conjugates) and overall stability in respective storage conditions. Casein
Buffer 20X-4X Concentrate 2 Antibody/Antigen/(PolyHRP)
Conjugate Diluent/Blocker Refer to PolyHRP Users Guide for learning in more details how can you benefit from better using our Diluents/Blockers and Stabilizers. |