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We currently offer PolyHRP conjugates in two liquid forms. One is concentrated stock solution for long term -20° storage. Another is pre-diluted in PolyHRP Stabilizer format for storage at +2°C/+8°C. Our standardized stock PolyHRP conjugate solutions are 1 mg/ml total chromatographically purified conjugate in cryopreserved liquid formulation containing 50% Ultra-pure glycerol, general protein stabilizer (Ultra-pure biotin-free BSA), special protohematin/HRP stabilizer and BND as biocide preservative. Cryopreserved stock PolyHRP conjugate solutions feature excellent stability when stored at -20°C in tightly closed vials. There are no strict requirements to packaging material when storing stock PolyHRP batches at -20°C. We currently have available data proving 100% activity maintenance with conjugate back-up samples stored up to 6-8 years at -20°C in both neutral/borosilicate type I glass, PET/PETG/PC (low oxygen permeability materials) and regular PP/PE-HD (oxygen permeable) bottles/vials. Cryopreserved stock conjugate solutions will remain liquid at temperatures down to -25°C. Stock solutions will freeze at temperatures below -25°C. Freezing conjugates at lower temperatures is not recommended. Neither of PolyHRP conjugates is filterable through 0.2m or smaller pore size filters. Re- filtering of concentrated stock PolyHRP conjugate solutions is not needed and not recommended. Re-filtering of pre-diluted and ready-to-use conjugate forms, if these are made of properly stored stock conjugate solution (or pre-diluted item) and PolyHRP Stabilizer of SDT at customers place in adequately clean conditions, when risk of contamination is minimized, is also not necessarily needed and not recommended. If you do wish to re-filter liquid formulation containing PolyHRP conjugate, use 0.45m- filters. This is the smaller filter pore size compatible with the PolyHRP. Best are the cellulose acetate filters with GF pre-filter mounted in single filter unit, e.g. 0.45 m MiniSart Plus of Sartorius. Do not use 0.2m filter units! See our PolyHRP Users Guide for more details on handling PolyHRP conjugates. Auxiliary products ideally compatible with PolyHRP conjugates are as follows: SA-PolyHRP line: ready-to-use products - Universal Casein Diluent/Blocker #UCDB - SA-PolyHRP Stabilizer #SA1 - SA-PolyHRP Stabilizer RHT (Realtime High Temperature) #SA3-RHT intermediate products - Casein Buffer Concentrate 1 #CBC1 Other (Protein A, Secondary Antibody & Custom) PolyHRP conjugates: ready-to-use - Universal Casein Diluent/Blocker #UCDB - SA-PolyHRP Stabilizer #SA1 - SA-PolyHRP Stabilizer RHT (Realtime High Temperature) #SA3-RHT - Antibody/Antigen/Conjugate Diluent/Blocker #AADB - Antibody/Antigen (PolyHRP) Conjugate Stabilizer #AA1 - Antibody/Antigen (PolyHRP) Conjugate Stabilizer RHT (Realtime High Temperature) #AA3-RHT intermediate - Casein Buffer Concentrate 1 #CBC1 - Casein Buffer Concentrate 2 #CBC2 |