AmpliStain™ 1-Step detection systems are composed of single reagents comprising StrongZyme™ Goat anti-Mouse, Goat anti-Rabbit and Rabbit anti-Goat conjugates made of specially engineered decarboxylated High Density Nude HRP™ di-, tri- tetra- and pentamers covalently coupled to the affinity purified anti-IgG(H+L) antibodies using proprietary SnakeLinker™ technology. Decarboxylation decreases hydrodynamic radius of HRP so that carbohydrate reduced Nude HRP™ molecules become smaller in size. Application of the long flexible linkers yields loose-jointed conjugate design. These factors make StrongZyme™ conjugates both exceptionally active in detection and good at penetration - they have very strong enzymatic signal-generating moiety and at the same time are compact, flexible and agile. StrongZyme™conjugates easily overcome diffusion limitations enabling very sensitive detection of the most challenging low-abundance and hidden nuclear markers. They feature enhanced detection activity with Mouse, Rabbit and Goat primary antibodies. In the direct comparisons AmpliStain™ 1-Step / StrongZyme™ is more sensitive than existent competitor's polymer detection systems. AmpliStain™ Essentials |
Comparison of AmpliStain™ #AS-M1-HRP with EnVision™+ in staining Ki-67 and KL1. AmpliStain™ still gives intensive staining with three times smaller working concentration of primary antibodies. |
StrongZyme™ and Ready to Use RESERVE™ are Trademarks of SDT.