10% BSA1, Ultra pure, biotin and IgG free, #BSA1
BSA1 is a 10% (w/v) solution of Receptor Grade Fraction V BSA in PBS
pH 7.3 with 1000 ppm 5-Nitro-5-bromo-1,3-dioxane (BND),
0.2µm-filtered.
It is certificated as product containing no traceable biotin and bovine IgG.
This product is stable 2 years when stored at +4°C.
Recommended use:
1) Blocking reagent for blocking ELISA immunoplates, blotting membranes and 3D porous
filter matrices after immobilization of capture antibody/antigen. Dilute 1/10 to 1/20 in
PBS/BND (made of #PBSC and water) to produce 1-0,5% blocking BSA solution.
2) Component of diverse diluent systems and other liquid IVD formulations - in the
capacity of general protein stabilizer and moderate NSB blocker.
3) 0,2% BSA1 diluted 1/50 in PBS/BND is the first choice
validated after-conjugate-wash-buffer-1 in 3D-PolyHRP/p(HS)TMB format assays.
BSA1 is universal unrestricted product fully compatible with
SA-PolyHRP in all listed above applications.
10% BSA, Ultra-pure, biotin and IgG free #BSA1
pricing
BSA2 is a 10% (w/v) solution of Fraction V BSA in PBS pH 7.3 with
1000 ppm BND, 0.2µm-filtered.
BSA2 may contain trace concentrations of biotin and bovine IgG.
Product is stable 2 years by +4°C.
Recommended as:
1) Blocking reagent for blocking ELISA immunoplates, blotting membranes and 3D porous
filter matrices after immobilization of capture antibody/antigen. Dilute 1/10 to 1/20 in
PBS/BND (made of #PBSC and water) to produce 1-0,5% blocking BSA solution.
2) Component of diverse diluent systems and other liquid IVD formulations - in the
capacity of general protein stabilizer and moderate NSB blocker.
3) 0,2% BSA2 diluted 1/50 in PBS/BND is the second choice after-conjugate-wash-buffer-
1 in 3D-PolyHRP/p(HS)TMB format assays.
BSA2 is not recommended for immediate use with SA-PolyHRP conjugates, i.e. as
component of SA-PolyHRP diluent system.
10% BSA, trace biotin and IgG
#BSA2
pricing
#HAMAB is supplied in liquid form as PBS solution containing (as per specification) 8 to
12 mg/ml mouse mieloma immunoglobulins G, mixture of different subclasses having no
identified antigen-binding activities, preserved with 1000 ppm BND, 0.2mm-filtered.
Reagent is stable 2 years (+2°/+8°C).
Recommended starting concentration for effective eliminating HAMA-associated false
positive (sandwich tests) and false negative (competitive tests) reactions is 200-250 µg/ml,
concentration range for optimization work is from 50 µg/ml to 1 mg/ml.
Blocker of Human Anti-Mouse IgG Antibodies (HAMA)
mixture of mouse mieloma IgGs (different subclasses, no
identified antigen-binding activity)
#HAMAB
pricing
1) Blocking Reagent - 1% BSA in PBS/BND:
1 Volume BSA1/2 + 0,9 (~1) Volumes #PBSC + 8,1 (~8) Volumes pure
deionized 0.1/0.2mm-filtered water
2) First after-conjugate Wash Reagent in 3D-ImmunoFiltration - 0,2% BSA:
1 Volume BSA1/2 + 4,9 (~5) Volumes #PBSC + 44,1 (~45) Volumes
pure deionized 0.1/0.2mm-filtered wate